26.5 Folate (categories 2)

Folate range (<40 vs >=40)

26.5.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 135.6600587 1 <0.0001 ***
Folate_gp2 4.6986120 1 0.03 *
Study 0.0222185 1 0.9 ns
GA_samp 126.0704095 1 <0.0001 ***
Folate_gp2:Study 0.5210428 1 0.5 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 31.5757792 1 <0.0001 ***
Folate_gp2 0.5739028 1 0.4 ns
Study 16.4561933 1 <0.0001 ***
GA_samp 1623.7970207 1 <0.0001 ***
Folate_gp2:Study 2.4557886 1 0.1 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 326.5768112 1 <0.0001 ***
Folate_gp2 0.2185473 1 0.6 ns
Study 0.6228831 1 0.4 ns
GA_samp 799.2090412 1 <0.0001 ***
Folate_gp2:Study 0.2208327 1 0.6 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp2 1 Inf 1.138 1.138 0.3 ns
3 Study 1 Inf 12.831 12.831 0.0003 ***
4 GA_samp 1 Inf 0.112 0.112 0.7 ns
2 Folate_gp2:Study 1 Inf 0.296 0.296 0.6 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp2 1 Inf 0.719 0.719 0.4 ns
3 Study 1 Inf 23.967 23.967 <0.0001 ***
4 GA_samp 1 Inf 0.182 0.182 0.7 ns
2 Folate_gp2:Study 1 Inf 0.544 0.544 0.5 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp2 1 Inf 0.835 0.835 0.4 ns
3 Study 1 Inf 64.705 64.705 <0.0001 ***
4 GA_samp 1 Inf 0.018 0.018 0.9 ns
2 Folate_gp2:Study 1 Inf 3.903 3.903 0.05 ns

26.5.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 13.72 (8.83, 21.34) <0.0001
Folate_gp2≥40 0.91 (0.84, 0.98) 0.01
StudySTOP 1.01 (0.89, 1.16) 0.85
GA_samp 1.16 (1.13, 1.20) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.94 (1.55, 2.43) <0.0001
Folate_gp2≥40 0.99 (0.95, 1.03) 0.48
StudySTOP 1.14 (1.07, 1.23) 0.0002
GA_samp 1.34 (1.32, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.06 (0.05, 0.08) <0.0001
Folate_gp2≥40 0.96 (0.91, 1.02) 0.20
StudySTOP 1.03 (0.94, 1.13) 0.52
GA_samp 1.32 (1.29, 1.34) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 16.05 (9.10, 28.32) <0.0001
Folate_gp2≥40 0.95 (0.85, 1.05) 0.31
StudySTOP 0.71 (0.60, 0.86) 0.0003
GA_samp 0.99 (0.96, 1.03) 0.73
HOMA-IR
Var Effect P
(Intercept) 3.96 (2.19, 7.18) <0.0001
Folate_gp2≥40 0.96 (0.86, 1.07) 0.45
StudySTOP 0.62 (0.51, 0.75) <0.0001
GA_samp 0.99 (0.96, 1.03) 0.66
Glucose (mmol/L)
Var Effect P
(Intercept) 5.47 (4.92, 6.07) <0.0001
Folate_gp2≥40 0.99 (0.95, 1.02) 0.49
StudySTOP 0.85 (0.82, 0.89) <0.0001
GA_samp 1.00 (0.99, 1.01) 0.89
Folate_gp2≥40:StudySTOP 1.05 (1.00, 1.10) 0.05

26.5.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 95.81370 7.927343 101.43210 81.31139 112.9026
STOP 97.08491 7.518415 78.25765 83.21432 113.2675
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 89.70208 2.651369 183.1901 84.62054 95.08877
STOP 102.61293 2.682940 112.2757 97.43251 108.06878
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.270916 0.0754325 410.8656 2.127372 2.424146
STOP 2.341951 0.0631302 207.2018 2.220741 2.469778
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.38027 0.9045247 Inf 12.712357 16.26702
STOP 10.27784 0.4593539 Inf 9.415826 11.21876
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.476492 0.2316803 Inf 3.050812 3.961566
STOP 2.146191 0.1003656 Inf 1.958223 2.352201
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.397599 0.0657130 Inf 5.270328 5.527943
STOP 4.720152 0.0407045 Inf 4.641043 4.800609

26.5.4 Comparisons (Overall)

Hormone Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) ≥40 / <40 0.91 (0.84, 0.98) 0.01
hPL (μg/ml) ≥40 / <40 0.99 (0.95, 1.03) 0.5
GH2 (ng/ml) ≥40 / <40 0.96 (0.91, 1.02) 0.2
Insulin (uU/ml) ≥40 / <40 0.95 (0.85, 1.05) 0.3
HOMA-IR ≥40 / <40 0.96 (0.86, 1.07) 0.5
Glucose (mmol/L) ≥40 / <40 1.01 (0.99, 1.04) 0.4

26.5.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

26.5.6 Plots